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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in several tumors, which results in chromosome instability, and so may add to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial part in advertising cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two further splice variants ended up described but in-depth nucleotide sequence info just isn't but obtainable.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This guide outlines further attainable optimization of mobile and immunoassay parameters to be certain the very best effects are...
Antibodies sure to the blocking peptide now not bind towards the epitope about the focus on protein. By comparing the staining through the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Info Cyclin E belongs to the highly conserved cyclin family, whose users are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and click here degradation patterns which lead for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and features being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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